Partner lab price : 48.99 €/hour
Public and private lab price: 131.03 €/hour

Imaging flow cytometer ImageStreamX MkII by Luminex. The system is controlled by the acquisition software INSPIRE. Analysis with the dedicated software IDEAS (Image Data Exploration Analysis Software)

3 LASERs : violet (405nm – 120mw), blue (488nm – 200mW), red (642nm – 150mW)
6 detection channels (Brightfield included) : 435-505 nm / 505-560 nm / 560-595 nm /595-642 nm / 642-745 nm / 745-785 nm
3 magnifications : 20x, 40x, 60x

Applications : Intracellular or cell-surface staining, fixed or alive cells to study cell signaling, cell cycle, internalization and co-localization, cell-cell interaction, shape change, immunological synapse, microbiology, parasitology…

Ideal operating temperatures between 20 and 24°C
L2 laboratory equiped with hood, fridge, centrifuge, pipets,

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : 17.47 €/hour
Public and private lab price: 82.24 €/hour

2D imaging

3D imaging

High Content Screening

Image analysis

Automated epifluorescent widefield microscope. Ideal for medium throughput image acquisition and analysis with a large number of samples (cellules, sphéroïdes...) fixed or live and in microplate format. Color and time-lapse imaging possible.
Piloted by CellReporterXpress

Objectives Leica:
4X/NA 0.13 FLUOTAR
10X/NA 0.32 FLUOTAR
20x/NA 0.40 FLUOTAR
40x/NA 0.60 FLUOTAR

Excitation Source:
6 LEDs : Cy5, TRITC, FITC, DAPI, Texas Red, CFP, white light (and RGB)

Filter cubes:
DAPI
FITC
TRITC
Cy5

Can read any format of microplate (6 à 384 wells) and conventional microscopy slides (x4)

Detector:
CMOS camera 5Mpx 12bits
(2000 x 2000 pixels image)

Environmental control: temperature, humidity and CO²

Applications:
-multidimensional automated acquisition: YXZ, multi-channels, multi-fileds, multi-wells, multi-plates, timelaps
-easy image analysis (on-the-fly): 25 preconfigured protocols: Cell counting, Multi-channel Cell scoring, Translocation, Neurite tracing...
-On-the-fly stitching and deconvolution (2D)
-hardware and image autofocus

L2 environment

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : -- €/hour
Public and private lab price: -- €/hour

High Content Screening

SBS plate reader ideal for 96/384 wells (6 - 1536 wells)

Equipped for fluorescence, luminescence and HTRF reading.

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : 5.36 €/hour
Public and private lab price: 22.32 €/hour

2D imaging

Videomicroscopy

Zeiss motorized widefield videomicroscope with incubation chamber for videomicroscpies in 2D with low resolution, reduced frequency, long timelapses in multi-positions, in fluorescence and transmission lights (Bright Field, Phase contrast)
Controlled by Metamorph software

Objectives:
5x Plan Neofluar 0.15 NA PH1
10X EC-Plan Neofluar 0.3 NA PH1
20X LD Plan Neofluar 0.4 NA PH2 Korr
32X LD A Plan 0.4NA PH1 + PLASDIC
40x LD Plan NEOFLUAR 0.6 NA PH2 Korr

Detector:
camera sCMOS Zyla 5.5 (Andor)
(2560*2160 pixels, 6.4µm pixel size)

Incubation:
control of temperature and CO2

Types of acquisition:
-epifluorescent widefield acquisition
-transmitted light acquisition in bright field mode or phase contrast

The Multi-dimensional Acquisition module in Metamorph controls the multi-series of images in multi-channels, multi-positions, multi-Z planes, multi-timepoints.

Filter cubes:
GFP, YFP, Cy3, Cy5

Illumination sources:
1 system Lumencor with 4 LEDs:
GFP, YFP, Cy3, Cy5
Halogen lamp 100W/12V for transmission

Compatible sample carriers::
multi-well plate in plastic, 10, 35, or 60mm diameter dish in plastic

L2 environment

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : 5.36 €/hour
Public and private lab price: 22.32 €/hour

2D imaging

Videomicroscopy

Inverted wide-field epifluorescence microscope ideal for 2D or 3D acquisitions followed by deconvolution, on live samples.

Fluorescence, phase and differential interference contrast (DIC) contrasts
Multi-dimensional acquisition
Environmental control (CO2, T°)

Acquisition software: Zen Blue

Objectives:
5X Fluar 0.25NA
10X EC Plan Neofluar 0.3 NA
10X LD A-Plan 0.25 NA Ph1
20X LD A-Plan 0.35 NA Ph1
20X LD Plan Neofluar 0.4 NA Ph2 Korr

- - -
Other objectives available on request :
10X N-Achroplan 0.25 NA Ph1
20X LD A-Plan 0.3 NA Ph1
40X LD Plan Neofluar 0.6 NA Ph2

Camera: Hamamatsu Orca flash 4.0 LT - B&W, 16-bit, 2048x2048 pixels, pixel size @6.5 µm

- - -
Supports:
Slides
35 mm Petri dishes
Culture dishes

- - -
Excitation sources:
Fluorescence: Colibri LED 375 nm, 470 nm, 555 nm
Transmitted light: LED

- - -
Filter cubes:
DAPI
GFP
Rhodamine

- - -
Types of acquisition:
Automated: XYZ, multichannel, mosaic
Time lapses with environmental control
Customizable protocol

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour

2D imaging

Videomicroscopy

Inverted Nikon TiE wide-field microscope for timelapse in 2D or 3D in fluorescence or transmitted light mode (BF, DIC). Trigger option for fast imaging.

Controled by NIS Elements.

Objectives:
10X Plan Apochromat 0.45NA air
20X Plan Apochromat 0.75NA air
40X Plan Fluor 1.3NA oil
60X Plan Apochromat 1.4NA oil
100X Plan Apochromat 1.40NA oil

Detectors:
1 camera sCMOS back-illuminated Prime95B Photometrics
(1200*1200, 11µm pixel size)

Incubation:
temperature and CO2 control

Type of acquisition :
-widefield epifluorescent microscopy
-transmitted light microscope in bright field or DIC mode.

PFS module for control of the z drift during timelapse.

Trigger option for fast multicolor imaging.

Illumination source:
Lumencor with 7 LED and excitation filters for:
DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5

Emission filters:
DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5

Compatible sample holders:
35mm dish with #1.5 glass bottom, glass slide with #1.5 glass coverslip or glass-bottom multi-well plate

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : 5.36 €/hour
Public and private lab price: 22.32 €/hour

2D imaging

Videomicroscopy

Olympus inverted widefield videomicroscope for timelapse in 2D with low resolution, reduced speed, long kinetics in multi-positions in epifluorescent or transmitted light (brightfield, phase contrast, like-DIC)

Controled by Metamorph

Objectives:
10X UPFLN 0.3NA PH1
20X LUCPLFLN 0.45NA PH1
20X LUCPLFLN 0.45NA RC2
40X LUCPLFLN 0.6NA PH2
40X LUCPLFLN 0.6NA RC2

Detector:
1 ZYLA 4.2 MP scMOS camera
(2048*2048 pixels, 6.5µm pixel size)

Incubation:
temperature and CO2 control

Type of acquisition :
-widefield epifluorescent microscopy
-transmitted light microscope in bright field, phase contrats or DIC-like mode.

The Multi-dimensional Acquisition module in Metamorph controls the multi-series of images in multi-channels, multi-positions, multi-Z planes, multi-timepoints.

Filter cubes:
Hoechst
FITC
Cherry
Cy5

Illumination sources:
White LED Xcite

Compatible sample carriers:
multi-well plastic plate; slide or bottom glass/plastic labtek, 35mm diameter disk bottom glass/plastic

L2 environment

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance