Leica SP8-UV confocal microscope
3D imaging
Videomicroscopy
FRAP photoactivation
Ratiométric FRET
DNA damage
Spectral imaging
Fluorescence (355, 405, Argon, 561 and HeNe 633 LASERs) and brightfield/DIC.
Multidimensional acquisitions (X,Y,Z, spectral and time), F-techniques, incubation
Partner lab price : 37.04 €/hour
Public and private lab price: 101.89 €/hour
Associated technologies:
Brief description:
State-of-the-art fully motorized, LAS software-driven, inverted confocal microscope (+UV) for epifluorescence and transmission imaging. Fluorescence (355, 405, Argon, 561 and HeNe 633 LASERs) and brightfield/DIC.
Multidimensional acquisitions (X,Y,Z, spectral and time), F-techniques, incubation
Objectives:
10X Plan Apochromat 0.4 NA Imm
20X Plan Apochromat 0.75 NA Imm Corr
40X Plan Apochromat 1.3 NA oil DIC
63X Plan Apochromat 1.4 NA oil DIC
63X Plan Apochromat 1.2W UVIS + water dispenser
Detectors:
2 cooled regular multialkaline PMTs
1 transmission PMT
3 cooled HyDs (GaAsP photocathode) for low noise photon counting imaging.
BSL1 Environment
10X Plan Apochromat 0.4 NA Imm
20X Plan Apochromat 0.75 NA Imm Corr
40X Plan Apochromat 1.3 NA oil DIC
63X Plan Apochromat 1.4 NA oil DIC
63X Plan Apochromat 1.2W UVIS + water dispenser
Detectors:
2 cooled regular multialkaline PMTs
1 transmission PMT
3 cooled HyDs (GaAsP photocathode) for low noise photon counting imaging.
BSL1 Environment
Illumination light source for widefield observation :
Fibred metal halide arc source for episcopic illumination
100W tungsten bulb for diascopic illumination
Motorization:
Binocular tube, X, Y and Z stage, fluorescence filter set turret, transmission mirror, objective turret
Fluorescence filter sets for widefield observation:
Hoechst
GFP
Rhodamine LP
Cy5
Fibred metal halide arc source for episcopic illumination
100W tungsten bulb for diascopic illumination
Motorization:
Binocular tube, X, Y and Z stage, fluorescence filter set turret, transmission mirror, objective turret
Fluorescence filter sets for widefield observation:
Hoechst
GFP
Rhodamine LP
Cy5
Available acquisition techniques:
Excitation with 355, 405, 458, 476, 488, 514, 561 & 633nm lines.
2/3D mosaic acquisition, spectral separation, timelapse analysis. In-focus repositioning.
Any photoactivation/destruction technique (FRAP, PA, FLIP), ratiometric FRET experiment, UVA induced DNA-damange on sensitized cells.
Screening and on the fly analysis (Matrix screener)
User-defined protocols
Excitation with 355, 405, 458, 476, 488, 514, 561 & 633nm lines.
2/3D mosaic acquisition, spectral separation, timelapse analysis. In-focus repositioning.
Any photoactivation/destruction technique (FRAP, PA, FLIP), ratiometric FRET experiment, UVA induced DNA-damange on sensitized cells.
Screening and on the fly analysis (Matrix screener)
User-defined protocols
Image quality:
Confocal technology allows improved spatial resolution and contrast compared to widefield imaging. Spatial resolution may be further improved using deconvolution algorithms (Huygens).
Sensitivity:
The HyD design allows better sensitivity and SNR compared to regular PMT and a less noisy photon counting imaging.
The fast resonant scanner decrease excitation-related toxicity.
Confocal technology allows improved spatial resolution and contrast compared to widefield imaging. Spatial resolution may be further improved using deconvolution algorithms (Huygens).
Sensitivity:
The HyD design allows better sensitivity and SNR compared to regular PMT and a less noisy photon counting imaging.
The fast resonant scanner decrease excitation-related toxicity.
Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.
