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Phone book

Contact / Function	Room	Facility	Mail	Phone
Lemaire Patrick Scientific leader of MRI			This email address is being protected from spambots. You need JavaScript enabled to view it.
Georget Virginie Operations manager of MRI	RDC141	CRBM	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 95 18 06 21 75 76 07
Faklaris Orestis Manager facility	RDC141	CRBM	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 95 18
De Rossi Sylvain Imagery / Development	RDC141	CRBM	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 95 18 06 98 31 85 94
Baecker Volker Manager MRI-CIA	CRBM RDJ034	MRI-CIA	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 95 68 06 50 19 27 42
Benedetti Clément Image analysis	CRBM RDJ034	MRI-CIA	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 95 68
Turpault Soumaya Imagery	RDC141	CRBM	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 95 18
Langlois Simon Imagery	RDC141	CRBM	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 95 18
Talignani Céline Manager Facility	1rst floor F2 building - room N1F3	IRCM	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 11 28 31 31
Gauron Carole Manager Facility	room 331	PHIV-DIADE	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 67 41 55 63
Bordignon Benoit Manager facility in Screening	CRBM RDJ018	CRBM-HCS	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 97 21
Hassen-Khodja Cédric Bioinformatician	CRBM RDJ018	CRBM-HCS	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 97 21
Diakou Vicky Manager facility	Campus Triolet - Building 24 2nd floor	DBS-opt	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 67 14 92 02 06 21 75 76 16
Jublanc Elodie Imagery	Campus Triolet - Building 24 2nd floor	DBS-opt	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 67 14 92 02
Blanchard Marie-Pierre Manager facility	SSol S02	IGH-opt	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 99 90
Cau Julien Imagery	SSol S02	IGH-opt	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 99 90
Sar Chamroeun Manager facility		INM	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 99 63 60 79
Hudecek Laetitia Imagery		INM	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 99 63 60 63
Mateos-Langerak Julio Imagery / Responsible R&D	SSol S02	IGH-opt	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 99 90
Alcon Carine Manager facility	2nd floor 229	PHIV-LaGaillarde	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 99 61 26 08
Dejou Cécile Manager facility	F2.2.06 (building 2, 2nd floor, last room)	IRCM-cyto	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 11 28 31 47
Boyer-Clavel Myriam Manager facility	SSol 004/0013	IGMM	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 96 90
Galtier Jérémy Manager facility / Imagery	SSol S02	IGH-opt IGH-cyto	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 99 90
Duperray Christophe Manager facility		IRMB	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 67 33 78 29
Leccia Felicia FLow cytometry		IRMB	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 67 33 78 29
Lebrun Renaud Manager facility	Campus Triolet - Building 22 RDC 54	ISEM	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 67 14 32 60
Viala Stéphanie Flow Cytometry	SSol 004/0013	IGMM	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 96 90
Miquel Olivier Manager MRI-NET	CRBM RDJ017	MRI-NET	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 96 73 06 50 19 27 43
Laborie Stéphane Computer park manager	CRBM RDJ016	MRI-NET	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 96 60 06 01 00 05 59
Goubet Dominique developer MRI-NET	CRBM RDJ034	MRI-NET	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 34 35 95 68
Dejean Matthieu Imaging/ Image analysis	RDC 7, 8, 44	PHIV-LaValette	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 67 61 57 94
Navarro Sanz Sergi Laser microdissection		PHIV-Lavalette	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 67 61 54 49
Daunas Lucien Electron Microscopy & image analysis	Campus Triolet - Building 24 4th floor	EM4Bio	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 67 14 93 16
Berry Laurence Facility Manager	Campus Triolet - Building 24 4th floor	EM4Bio	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 67 14 93 16
Belec Salomé Sample prepraration	Campus Triolet - Building 24 4th floor	EM4Bio	This email address is being protected from spambots. You need JavaScript enabled to view it.	04 67 14 93 16

Posted in Phone book

PRESENTATION

Montpellier Ressources Imagerie is a multi-site Technological Facility of UAR Biocampus, certified ISO 9001-2015 NFX 50-900 and member of the national infrastructure France-BioImaging. The management of MRI is done by Patrick Lemaire as scientific director and Virginie Georget as operational director.

MRI offers equipments and expertise in light microscopy, electron microscopy, screening, RX Tomography and flow cytometry, as well as in image analysis and software development to support you in your scientific projects in life biology. The facility is open to public and private sector without any thematic, institutional or geographic conditions.

After registration and training by MRI engineers, users can book the equipment online wherever they are.

In case of publications of data acquired on MRI facilities, we asked our users to aknowledge the MRI facility with the following sentence: "We acknowledge the imaging facility MRI, member of the France-BioImaging national infrastructure supported by the French National Research Agency (ANR-10-INBS-04, «Investments for the future»)"

When MRI engineers is co-authors on publications, we ask that their affiliation be:

1) Montpellier Ressources Imagerie, BioCampus, University of Montpellier, CNRS, INSERM, Montpellier, France

and if he/she is assigned to a research unit other than BioCampus, then a second affiliation must appear:

2) "assignment unit acronym", University of Montpellier, "les tutelles de l'unité", Montpellier, France.

Our Scientific Guardians and Institutional Partners

237px CNRS fr quadri.svg logo generique SD LOGO original RVB petit

logo cirad transp logoMSA2014 vert Web

Labs supporting MRI

CRBM IGH IGMM INM IGF CBS DBS-UM IPSiM AGAP ISEM DMEM LPHI IRMB IRCM

Our quality pledges

ISO 9001 and NFX 50 900 ibisa

Our current ISO9001 and NFX50-900 certification scope concerns "the provision of instruments, expertise and realization do services and collaborative projects for scientific imaging (light microscopy, flow cytometry, screening, X-ray tomography"

ORGANISATION CHART

SCIENTIFIC POLICY

BILLING AUTHORIZATION

Posted in Presenting

Presentation

Our services offered to the scientific community are organized into two services: facility operations (machines, users, account ...) and a software development team.

STAFF

Olivier Miquel

MRI-NET
Manager

Stéphane Laborie

MRI-NET
Computer park manager

OPERATIONS (MRI-NET)

MRI-NET provides operational missions, development of the IT infrastructure and user support.

The role of MRI-NET is to follow the operating positions, maintain and integrate them into technical platforms according to their local security rules established by the agencies.

MRI-NET provides assistance to users and engineers.

MRI-NET manages registrations, credit groups, the establishment of pricing in the counting system of "time of use" and assists users in managing their account.

MRI-NET team also maintains the databases, software developments, fixing bugs and produces software based on user requirements.

HOW TO FIND US?

CRBM UMR5237

1919 route de Mende
34293 Montpellier Cedex 05

Téléphone MRI-NET : +33 4 34 35 96 73 / 96 60
Téléphone gestion : +33 4 34 35 95 68
Téléphone MRI-DEV : +33 4 34 35 95 18

GPS : Latitude = 43.63767 ; Longitude = 3.86544

Posted in Our IT resources

Principle of Flow cytometry (CMF)

Flow cytometry allows the study of isolated, in suspension cells carried by a liquid vector. With this technique, you obtain an individual, qualitative and quantitative caracterisation of cells.

Each particle, carried by the liquid, passes through a laser beam and the cytometer collects the optical or physical signals. The provided signals are relative to :

-the optical properties linked to the laser light scatter (size, intracellular complexity)

-the optical properties linked to the emitted fluorescence due to specific staining of structures or cellular fonctions.

This individual analysis (cell by cell) is multiparametric and can be done at the speed of several thousand events per sec.

An application of flow cytometry is cell sorting that allows the physical separation of one or several cells populations.

Posted in Principles and fluorochromes

R & D

Posted in R & D

Ratiometric FRET

Posted in Our technologies

RICS

Posted in Our technologies

Sample preparation

-Automates pour la Microscopie électronique à balayage :

°Point Critique CO2 : CPD030 Baltec : Photo + petit texte

°Métalliseur ou Sputter Cutter : SC500-POLARON : Photo + petit texte

-Automates pour la Microscopie électronique à transmission :

°Inclusion en résine pour des études de morphologie : AMW Leica : Photo + petit texte

°Inclusion à froid pour immunomarquage et CLEM : AFS Leica : Photo + petit texte

°Contraste de grilles par coloration : AC20 Leica : Photo + petit texte

°Immunogold sur coupe : IGL Leica : Photo + petit texte

-Coupe ultrafines : ULTRA-MICROTOME

°Reichert UltraCut S Leica 1 : photo

°Reichert UltraCut S Leica 2 : photo

Posted in Electronic microscopy

Scanner Pannoramic 3DHistech

Partner lab price : 17.47 €/hour
Public and private lab price: 82.24 €/hour

Associated technologies:

2D imaging

Cuts acquisition automating

Brief description:

Auomated scanner to produce virtual images from histological slides (brightfield and fluorescence).

Automatic slider for 12 slides

Objectifs :
20X NA 0.8 Apo
40X NA 0.95 Apo

Filter:
Quad band- DAPI ex 367-390 em 414-450
Quad band- FITC ex 461-487 em 500-530
Quad band Alexa 546 ex 544-564 em 564-584
Quad band- CY5 ex 626-644 em 660-799
Mono band Alexa 594 ex 570-590 em 661-690
Mono band Cy7 ex 670-745 em 768-849

Multiled excitation source

Detector:
pco edge back illuminated sCMOS camera (pixel size 6.5µm)

Automated slider for 12 slides

Length 75.0 to 76 mm/ lamella max 50 mm
Width 25.0 to 26.0 and 51.0 to 52.02 mm
Thickness 0.9 to 1.2 mm
45° bevelled corners

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Posted in Automated imaging

Scanner Zeiss AxioScan

Partner lab price : 17.47 €/hour
Public and private lab price: 82.24 €/hour

Associated technologies:

2D imaging

Brief description:

High Throughput Imaging dedicated to immunostaining (color) or immunofluorescence for histology. Automatic loading of slides. Essential system for toxicity studies and high content screening. Storage virtualization after staining and immunostaining.

Objectifs :
20X/0.8NA
40X/0.95NA

Cameras:
colored camera : Hitachi 3CCD
black and white camera: Hamamatsu orca flash

Illuminationsource for fluorescence:
colibri2 led (405nm, 488nm;, 555nm, 639nm)

Filter cubes:
eGFP, Dapi, CY3, CY5

Acquisition mode
automatic acquisition (100 slides)
immuno- and immuno-fluorescence histology