SPIM: Lightsheet Z.1


Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour
Associated technologies:
  • 3D imaging
  • Videomicroscopy
  • In vivo imaging

    • Brief description:
    Light Sheet microscope Z.1 Zeiss for fast acquisitions of live samll organisms like embryons ou cleared samples. The microscope is equipped with one illumination arm and one detection arm with one 20X objectives and 2 cameras.

    Controlled by Zen software
    Objectives:
    20X / water n=1.45
    20X clearing objective
    5X clearing
    Détectors:
    PCO.Edge, sCMOS, 1920 × 1920 pixels

    Illumination sources:
    laser 405nm
    laser 488nm
    laser 561nm
    Spectral selection:
    4 dual band emission filters in a filter wheel
    DAPI-GFP
    DAPI-Cy3
    GFP-Cy3
    GFP-DRAQ5

    Incubation:
    temperature between 10°C and 40°C - CO2 control possible
    2 incubation chambers: a classical one for fixed or live samples and one for cleared sample
    Acquisition mode
    -multi-dimensional light-sheet acquisitions: multi-channels, multi-Z stacks, multi-timepoints, multi-angles with rotation of the sample up to 360°
    Sample carriers:
    the sample is prepared in a glass capillary either embedded in a gel or loaded in a well formed in the gel. This capillary is charged in the sample carrier on the stage.
    Each project done on this Light Sheet needs specific development for the sample preparation