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Upright 2 Zeiss Apotome


Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour
Associated technologies:
  • 2D imaging
  • 3D imaging

  • Brief description:
    Upright widefield epifluorescence microscope ideal for 2D or 3D acquisitions followed by deconvolution, for fixed samples between slide and coverslip.

    Fluorescence and brightfield/darkfield
    Differential interference contrast (DIC)
    Multi-dimensional acquisition

    Acquisition software: ZEN Blue 3.2
    Objectives:
    5X EC Plan Neofluar 0.15 NA
    10X EC Plan Neofluar 0.3 NA
    20X Plan Apochromat 0.8 NA
    40X EC Plan Neofluar 1.3 NA oil
    63X Plan Apochromat 1.4 NA oil
    100X alpha Plan Apochromat 1.46 NA oil
    Camera: Hamamastu Orca flash 4.0 - B&W, 16-bits, 2048x2048 pixels, pixel size @6.5 µm
    - - -
    Excitation source: HXP120V Lamp

    - - -
    Filter cubes:
    Hoechst
    GFP
    Cy3
    Texas Red
    Cy5
    FluorGold
    - - -
    Type of acquisition:
    Automated on a slide: XYZ, multichannel, mosaic
    Customizable protocol
    
    Mouse spine - MRI 2007 Image Contest 2nd prize
    Transverse section of mouse spine at P0 stage, with spinal cord in the center and dorsal root ganglia on either side: Ret (Magenta), NF200 (Cyan).
    Steve Bourane, Institute for Neurosciences of Montpellier (INM), Montpellier

    Upright 4 Zeiss AxioImager Z2

    
    Partner lab price : 15.66 €/hour
    Public and private lab price: 55.41 €/hour
    Associated technologies:
  • 2D imaging
  • 3D imaging

  • Brief description:
    Epifluorescent upright widefield microscope dedicated to 2D and 3D acquisition conjugated to deconvolution for fixed samples between slide and coverslip.
    Fluorescence -Brightfield and Darkfield
    DIC
    Multi-dimensional acquisition

    Controlled by Zen software
    Objectives:
    10X EC Plan Neofluar 0.3 NA
    20X Plan Apochromat 0.8 NA
    40X EC Plan Neofluar 1.3 NA oil
    63X Plan Apochromat 1.4 NA oil
    100X Plan Apochromat 1.4 NA oil
    Excitation Source:
    Xcite 120LED Boost

    Filter cubes:
    Hoechst
    CFP
    GFP
    YFP
    Cy3
    Texas Red
    Cy5
    Cy7
    Detector:
    1 scMOS ZYLA 5.5 camera
    (2560 x 2160 pixels, 6.5µm pixel size)

    Acquisition mode:
    automatisation mode: XYZ, multi-channels, scan slide
    custom-made protocol

    Continue Reading

    Upright 5 Zeiss Axioimager Z2

    
    Partner lab price : 15.66 €/hour
    Public and private lab price: 55.41 €/hour
    Associated technologies:
  • 2D imaging
  • 3D imaging

  • Brief description:
    Epifluorescent upright widefield microscope dedicated to 2D and 3D acquisition conjugated to deconvolution for fixed samples between slide and coverslip.
    Fluorescence -Brightfield and Darkfield
    DIC
    Multi-dimensional acquisition

    Controlled by Metamorph software
    Objectives:
    10X EC Plan Neofluar 0.3 NA
    20X Plan Apochromat 0.8 NA
    40X EC Plan Neofluar 1.4 NA oil
    63X Plan Apochromat 1.4 NA oil
    100X Plan Apochromat 1.46 NA oil
    Excitation Source:
    HXP120 lamp
    auto-centred lamp



    Filter cubes:
    Hoechst
    CFP
    GFP
    YFP
    DsRed
    Texas Red
    Cy5
    Detector:
    1 scMOS ZYLA 4.2 MP camera
    (2048 x 2048 pixels, 6.5µm pixel size)
    Acquisition mode:
    automatisation mode: XYZ, multi-channels, scan slide
    custom-made protocol

    Continue Reading

    Upright Leica Thunder

    
    Partner lab price : 15.66 €/hour
    Public and private lab price: 55.41 €/hour
    Associated technologies:
  • 2D imaging
  • 3D imaging

  • Brief description:
    Epifluorescent upright widefield microscope dedicated to 2D and 3D acquisition conjugated to denloising and deconvolution for fixed samples between slide and coverslip.
    Fluorescence -Brightfield and Darkfield
    DIC
    Tile-scan

    Controlled by LAS X
    Objectives:
    4X Plan Fluotar 0.13 NA
    10X Plan Apochromat 0.45 NA
    20X Plan Apochromat 0.8 NA
    40X Plan Apochromat 0.95 NA
    40X Plan Apochromat 1.3 NA oil
    63X Plan Apochromat 0.6-1.4 NA oil
    100X Plan Apochromat 0.7-1.4 NA oil
    Excitation Source:
    LED3 Leica


    Filter cubes:
    Hoechst
    GFP
    Cy3
    Texas Red
    Cy5
    Detector:
    1 camera sCMOS Leica DFC9000 GT USB3
    (2048 x 2048 pixels, 4.2MP, 6.5µm pixel size)

    1 color camera Leica K3C (3072x2048 pixels, pixel size 2.4µm)
    Acquisition mode:
    Multidimentional acquisition : 2D, 3D+time multicolor
    Navigator Module for fast and easy mosaique, multiposition and focus correction
    quality and resolution improvement in real time or post traitement with Leica THUNDER technology

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    Welcome

    6 December 2024 : MRI Symposium : Focus on the new technologies
    The next MRI Day is scheduled for Friday 6 December at Genopolys. We would like to thank the speakers who have agreed to share their science and illustrate their experience with MRI. The focus will be on the latest technologies installed on MRI. You will find all the information on the poster.

    Don't forget to register before 31 October here.

     

    October 2024 : New confocal microscope in the new MRI-PHIV-DIADE facility
    A new facility has been set up at the IRD within the UMR DIADE. It is run by Carole Gauron, who manages an AxioZoom macroscope and a new Leica confocal microscope, a Stellaris 8. All the details are on the dedicated webpage.

    Thanks to contact This email address is being protected from spambots. You need JavaScript enabled to view it. for more informations and to discuss your projects

     

    October 2024 : MRI-ISEM at the Fête de la Science
    Renaud Lebrun, manager of the MRI-ISEM X-Ray tomography facility and his colleague Mavea Orliac will be welcoming you on saturday 5 october for the Fête de la science. They'll be telling you all about the evolution of cetaceans. Don't hesitate to go and see them

     

    October 2024 : MRI present to the first meeting of BioCampus experts
    The MRI facility had the opportunity to present its activities and expertise at the morning session organised by the UAR BioCampus on the theme of ‘Pre-clinical models and alternatives’. The main MRI help in this area is to characterise and screen the models proposed by the RAM, ZEFIX, POM and DROSO facilities.

    For more information, please contact us at This email address is being protected from spambots. You need JavaScript enabled to view it.

     

    September 2024 : MRI present at Phot'Aubrac
    As a follow-up to the 2023 photo exhibition ‘La vie est belle’, MRI exhibited a selection of photos at the Phot'Aubrac. festival. As at the Jardin des Plantes last year, the exhibition was a great success with the general public and we enjoyed explaining our professions. An experience worth repeating!

     

    September 2024 : the MRI-EM4Bio platform present to the CRYO-EMontpellier symposium
    Laurence Berry, head of the new MRI-EM4bio platform, had the opportunity to present her technological solutions during the day dedicated to CRYO-EM. Feel free to see what was on offer on the dedicated webpage.

    Please contact This email address is being protected from spambots. You need JavaScript enabled to view it. for more information or to discuss your projects.

     

    June 2024 : New confocal microscope in the MRI-INM facility
    The MRI-INM facility offers access to a new Leica confocal microscope. This is a Stellaris 5 equipped with a white laser, hybrid detectors and 6 objectives, including 2 long-distance objectives. Full description of this machine on the dedicated webpage

    Please contact This email address is being protected from spambots. You need JavaScript enabled to view it. for more information or to discuss uour projects

     

    June 2024 : MRI present to the Occitanie Réseau Imagerie meeting in Perpignan
    Like every year, MRI took part in the Occitanie imaging network day. This year, an expedition was organised to join our colleagues from Toulouse, Perpignan and Banyuls-sur-mer in Perpignan. It was a day rich in scientific and professional exchanges! See you next year.

     

    May 2024 : MRI present to the ANF ApiPhot - Assises RTmFm
    Few MRI engineers attended the APiPhot training course organised to mark the 20th anniversary of our national RTmFm network. Orestis Faklaris and Julio Mateos-Langerak presented the work they are involved in as part of RTmFM or FBI working groups. MRI is also present in the Microscopoly created by the RTmfm!

     

    April 2024 : MRI is looking for an engineer in Flow cytometry and Photonic microscopy
    We are looking for an engineer who will spend his/her working time between 2 assignments within the MRI facility: facility manager for the IGH cytometry facility and work as an cytometry engineer and microscopy engineer on the IGH microscopy facility. The fixed-term contract starts on 15 June 2024 for one year and is renewable. The profile is available on the CNRS job portal:ici

    Please, feel free to contact This email address is being protected from spambots. You need JavaScript enabled to view it. or This email address is being protected from spambots. You need JavaScript enabled to view it. for more informations.

     

    March 2024 : MRI is looking for a technician for Electron Microscopy
    We are looking for a technician dedicated to the sample preparation in electron microscopy. The fixed-term contract will run from as soon as possible until 31/12/2025. This person will work on the new MRI-EM4Bio facility recently created in building 24 on the Triolet Campus of the University of Montpellier. The profile is here.

     

    2024 : Our training shedule for the year:
    MRI has scheduled all its training activities (BioCampus workshops). These courses are given in french. The dates are as follows:

    Microscopie à épi-fluorescence et microscopie confocale : de la base à la pratique : 4-7 march
    Cytométrie en flux: de la préparation des échantillons à l’analyse des données: 19-20 march
    Programmation des macros avec Image J: 26-28 march
    Les bases du logiciel Image J : 25,26 et 29 april
    L’apprentissage automatique (machine learning)appliqué à l’analyse d’images biologiques : 4-6 june
    Imagerie avancée : Haute résolution en microscopie photonique : 26-26 june
    Analyse d’images 3D : 8-10 october
    Formation FlowJo niveau débutant : 7 november

     

    January 2024 : Best wishes :
    MRI wishes you all a very happy new year 2024. As every year, MRI staff will be on hand to provide you with the best possible support for your research projects.

     

     

    9-17 november 2023 : MRI at Mifobio :
    6 MRI engineer and the MRI scientific director attended the latest edition of the Mifobio 2023 theme school on the Giens peninsula. An ideal opportunity to learn about the latest technological advances and an excellent way to share our experience through workshops and round tables.

     

    october 2023 : MRI video online! :
    As part of its 20th anniversary celebrations, MRI has produced a new video of the facility, looking backwards and forwards.

    In preview!

     

    17 - 19 octobre 2023 : Symposium "Imagine Life : the Future" :
    As part of its 20th anniversary celebrations, MRI organised a symposium at the Corum in Montpellier. This 2.5-day symposium aims to examine how future developments in light/electron microscopy and image analysis could further transform research in animal and plant biology at multiple scales, from molecules to tissues. The symposium will include 7 thematic sessions (image analysis, cancer biology, plant biology, 3D genome architecture and transcription, neurosciences, cell and developmental biology, infection and immunity).

     

    29 september - 31 october 2023 : Photo exhibition "La vie est belle"
    MRI is organising a photo exhibition of 44 large-format science photos entitled "La Vie est belle!" as part of its 20th anniversary celebrations. This exhibition is open at the Montpellier Jardin des Plantes (Blvd Henri IV) until 31 October. This exhibition combines 32 scientific photos taken on the MRI platform and communicated by its users and 12 ambient photos, which show the process of capturing these images.

    Entrance to the exhibition is free and the venue magical. Don't miss the exhibition and come along with your family or friends: you don't have to be a scientist to enjoy it!

     

    21 september 2023 : Seminar by Laurence Berry about the new MRI-EM4Bio electron microscopy facility
    On the occasion of the creation of an electron microscopy service on MRI, we are pleased to announce a seminar to be held on 21 September at 9.45am in room sc36.09 of the Triolet Campus (new white building at the entrance to the University). Laurence Berry, who is in charge of the facility, will explain the range of technologies and applications we can offer you, based on the use of the microscope recently installed in building 24, the Zeiss Gemini 360 and the volutome. This microscope was funded as part of the European ReactEU program.

    A dedicated webpage is currently under contruction.

     

    Zeiss Axio-Observer

    
    Partner lab price : 5.36 €/hour
    Public and private lab price: 22.32 €/hour
    Associated technologies:
  • 2D imaging
  • 3D imaging
  • Videomicroscopy

  • Brief description:
    Motorized widefield inverted microscope with an incubator ("Videomicroscope") for timelapse imaging. The workstation is controlled through ZEN software.
    For epifluorescence and transmission imaging (brightfield, Phase and PLASDIC techniques).
    Multidimensional acquisition (X,Y, Z, lambda & t)
    Objectives:
    5x Plan NEOFLUAR 0.16 NA PH1
    10X A-Plan 0.25 NA PH1
    20X Plan NEOFLUAR 0.4 NA PH2 Korr + PLASDIC
    40X A-Plan 0.5 NA PH2 PLASDIC
    40x Plan NEOFLUAR 0.6 NA PH2 Korr
    63X Plan Apochromat 1.4 NA oil

    Detector:
    1 Hamamatsu ORCA-Flash4 monochrome camera
    (2048 x 2048 pixels, 6.5µm pixel size)

    BSL1 environment
    Illumination:
    XCite 120LED controlled by Zen for episcopic illumination
    100W tungsten filament bulb for diascopic illumination

    Motorization:
    Binocular tube, X, Y and Z stage, fluorescence filter set turret, objective turret.

    Fluorescence filter sets:
    Hoechst
    FITC
    Cy3
    Cy5
    CFP
    Available acquisition techniques:
    Any 2 or 3D acquisition along time (ranging from a few hours to a few weeks) with any compatible supports such as microscope slide, petri dish or culture plate, with both fluorescence & transmission modes (brightfield, phase contrast, PLASDIC).
    Mosaic acquisition/reconstruction of a full well in both fluorescence & transmission modes.

    User-defined protocols.
    For any inquiries, please contact This email address is being protected from spambots. You need JavaScript enabled to view it.

    Zeiss Axioimager M2 Apotome2

    
    Partner lab price : 15.66 €/hour
    Public and private lab price: 55.41 €/hour
    Associated technologies:
  • 2D imaging
  • 3D imaging

  • Brief description:
    Upright widefield microscope for 2D or 3D acquisition dedicated to fixed samples between slide and coverslip
    B&W Caméra CMOS Orca Flash 4.0
    XYZ motorized stage
    Fluorescence, Brightfield,
    Apotome 2 module

    Software : Zen by Zeiss
    Objectives:
    10X Plan Apochromat / 0.45 NA
    20X Plan Apochromat / 0.8 NA
    40X Plan Apochromat 1.3 NA. oil
    63X Plan Apochromat 1.4 NA. oil
    100X Plan Neofluar 1.3 NA. oil
    Illumination source for fluorescence : HXP-120

    Fluorescence filter cubes:
    Dapi. Ex : 335-383. Em :420-470
    Alexa Fluor 489 Ex : 450-490. Em :515-565
    DsRed Ex : 533-558. Em :570-640
    HE mPlum Ex : 574-599. Em :612-682
    Cy5 Ex : 625-655. Em :665-715
    Detector:
    B&W Caméra CMOS Orca Flash 4.0 (Hamamatsu) for large field and high resolution (2048 x 2048; pixel size 6.5µm)
    Apotome 2 module