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Thunder Leica


Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour
Associated technologies:
  • 2D imaging
  • 3D imaging

  • Brief description:
    Epifluorescence upright microscope
    motorized stage in XYZ
    Quality and resolution improvement with Leica THUNDER software technology
    suitable for thick and photosensitive samples
    Objectives :
    1,25X Plan Fluotar 0.04 NA (WD 3.7)
    5X Plan Fluotar 0.15 NA (WD 13.7)
    10X Plan Fluotar 0.32NA (WD11.13)
    20X Plan Fluotar 0.55 NA (WD 1.2)
    40X Plan APo 1.3 NA (WD 0.17)
    63X Oil Plan Apo 1.4 NA (WD 0.14)
    Illumination source Led for Transmission and Fluorescence

    Filter cubes:
    Dapi ex340-380 em 450-490
    GFP ex 450-490 em 500-550
    RHO ex 530-560 em 565-605
    Cy5 ex 590-650 em 662-738
    B & W Camera for Fluorescence
    Camera Monochrome Leica DFC 9000 GT ; (2048 x 2048 pixel size 6.5 µm)
    Software : LAS X
    Multidimentional acquisition : 2D, 3D+time
    Navigator Module for fast and easy mosaic, multiposition and focus correction
    quality and resolution improvement in real time or post processing with Leica
    THUNDER technology :
    - Instant Computational Clearing (ICC in 2D) = real time identification of out of focus signal, debluring
    - Large Volume Computational Clearing (LVCCS)
    Preserve raw data, quantifiable, adapted for thick and photosensitive samples

    TIRF PALM STORM

    
    Partner lab price : 17.47 €/hour
    Public and private lab price: 82.24 €/hour

    Brief description:
    Nikon widefield inverted microscope for epifluorescence and TIRF acquisition for 2D and 3D live imaging coupled to the FRAP/photo-activation/photoconversion technics. This microscope is also dedicated to the super-resolution technics (2D-3D) as PALM and STORM.
    Controlled by Metamorph software
    Objectives:
    10X Plan Apochromat 0.45NA air
    20X Plan Apochromat 0.75NA air
    60X Plan Apochromat 1.4NA oil
    100X Plan TIRF Apochromat 1.49NA oil

    Detectors:
    1 EMCCD iXon 897 Ultra Andor camera
    (512*512, 16µm pixel size)


    Incubation:
    Temperature and CO2 control.
    Acquisition thanks to the Roper iLAS2:
    -widefield microscopy
    -multi-colour TIRF microscopy
    -FRAP/photo-activation/photo-conversion in single point, predefined ROI or on the fly
    -PALM or STORM super-resolution

    The Multi-dimensional Acquisition module in Metamorph controls the multi-series of images in multi-channels, multi-positions, multi-Z planes, multi-timepoints.
    Illumination sources:
    1 Lumencor with 7 LED and excitation filters for:
    DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5
    1 laser bench with:
    405nm (100mW), 488nm (150mW), 561nm (500mW) et 642nm (1W)

    Spectral specificities:
    -EMCCD camera: Hoechst, GFP, mCherry, Cy5 with laser excitation or GFP and Cherry with LED excitation
    -sCMOS camera: Hoeschst, CFP, GFP, Cy3, mCherry with LED excitation
    Compatible sample carriers:
    35mm dish with #1.5 glass bottom, glass slide with #1.5 glass coverslip or glass-bottom multi-well plate

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    Tools

    For MRI users with a username and password:

    •    Registration for the MRI platform
    To use the facilities of the platform, it is first necessary to register and monitor the conditions of entry which will be sent by email.

    •    Password Loss
    In case of password loss, enter your email and you can change your password.

    •    Change your email account in your MRI
    Log in and click "manage my account"

    •    Booking system equipment
    Schedule management system equipment. To book equipment, you must be registered to the platform and have a username and password.

    •    MRI GLPI
    This software can report incidents on the MRI machine platform (microscopes, cytometers, tomograph RX)

    •    File management: SFTP server (see installation guide)

    This interface allows you to manage your files, transfer them to your account or download them. The images will deleted after 2 years automatically.

    •    OMERO
    This is a centralized software to manage your images. You can manipulate, share and store the images from this software.

    •    GLPI for MRI-NET
    This software allows to report incidents or requests computer.

    •    Redmine
    This software helps manage projects of the platform, some projects are public and some are private and not accessible to unauthorized persons.

    Internally for facilities responsibles:

    •    GLPI for MRI-NET
    This software allows to report incidents or requests computer.

    •    Dysfunction Editor

    This application is used to declare and manage malfunctions on equipment in conjunction with the booking system.

    •    Autonomy Editor 

    This application allows you to manage user skills on equipment in conjunction with the booking system.

    •    Leave
    Monitoring of staff leave the MRI platform.

    •    Quality
    Document management system for quality

    •    Owncloud
    Document management and online calendar for tracking quality of the platform. Sharing quality documents.

    •    Webmail
    Our e-mail management system for staff with an address "mri.cnrs.fr".

    Upright 2 Leica

    
    Partner lab price : 15.66 €/hour
    Public and private lab price: 55.41 €/hour
    Associated technologies:
  • 2D imaging
  • 3D imaging

  • Brief description:
    Epifluorescent upright widefield microscope dedicated to 2D and 3D acquisition for fixed samples between slide and coverslip.
    Fluorescence -Brightfield
    DIC
    Tile-scan

    Controlled by LAS X
    Objectives:
    10X Plan Apochromat 0.45 NA
    20X Plan Apochromat 0.8 NA
    40X Plan Apochromat 0.95 NA
    40X Plan Apochromat 1.3 NA oil
    63X Plan Apochromat 0.6-1.4 NA oil


    Detector:
    1 camera sCMOS Leica K5
    (2048 x 2048 pixels, 6.5µm pixel size)

    Excitation Source:
    Lamp HXP120



    L1 environment
    Filter cubes:
    Hoechst
    GFP
    Cy3
    Texas Red
    Cy5

    Acquisition mode:
    Multidimentional acquisition : 2D, 3D+time multicolor
    Navigator Module for fast and easy mosaique, multiposition and focus correction

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