English
Super resolution 3D SIM
Super-Resolution
Technology workshop
Technology workshop
Thunder Leica
motorized stage in XYZ
Quality and resolution improvement with Leica THUNDER software technology
suitable for thick and photosensitive samples
1,25X Plan Fluotar 0.04 NA (WD 3.7)
5X Plan Fluotar 0.15 NA (WD 13.7)
10X Plan Fluotar 0.32NA (WD11.13)
20X Plan Fluotar 0.55 NA (WD 1.2)
40X Plan APo 1.3 NA (WD 0.17)
63X Oil Plan Apo 1.4 NA (WD 0.14)
Filter cubes:
Dapi ex340-380 em 450-490
GFP ex 450-490 em 500-550
RHO ex 530-560 em 565-605
Cy5 ex 590-650 em 662-738
Camera Monochrome Leica DFC 9000 GT ; (2048 x 2048 pixel size 6.5 µm)
Multidimentional acquisition : 2D, 3D+time
Navigator Module for fast and easy mosaic, multiposition and focus correction
quality and resolution improvement in real time or post processing with Leica
THUNDER technology :
- Instant Computational Clearing (ICC in 2D) = real time identification of out of focus signal, debluring
- Large Volume Computational Clearing (LVCCS)
TIRF
TIRF PALM STORM
Controlled by Metamorph software
10X Plan Apochromat 0.45NA air
20X Plan Apochromat 0.75NA air
60X Plan Apochromat 1.4NA oil
100X Plan TIRF Apochromat 1.49NA oil
Detectors:
1 EMCCD iXon 897 Ultra Andor camera
(512*512, 16µm pixel size)
Incubation:
Temperature and CO2 control.
-widefield microscopy
-multi-colour TIRF microscopy
-FRAP/photo-activation/photo-conversion in single point, predefined ROI or on the fly
-PALM or STORM super-resolution
The Multi-dimensional Acquisition module in Metamorph controls the multi-series of images in multi-channels, multi-positions, multi-Z planes, multi-timepoints.
1 Lumencor with 7 LED and excitation filters for:
DAPI, CFP, GFP, YFP, Cy3, Cherry, Cy5
1 laser bench with:
405nm (100mW), 488nm (150mW), 561nm (500mW) et 642nm (1W)
Spectral specificities:
-EMCCD camera: Hoechst, GFP, mCherry, Cy5 with laser excitation or GFP and Cherry with LED excitation
-sCMOS camera: Hoeschst, CFP, GFP, Cy3, mCherry with LED excitation
35mm dish with #1.5 glass bottom, glass slide with #1.5 glass coverslip or glass-bottom multi-well plate
Tools
For MRI users with a username and password:
• Registration for the MRI platform
To use the facilities of the platform, it is first necessary to register and monitor the conditions of entry which will be sent by email.
• Password Loss
In case of password loss, enter your email and you can change your password.
• Change your email account in your MRI
Log in and click "manage my account"
• Booking system equipment
Schedule management system equipment. To book equipment, you must be registered to the platform and have a username and password.
• MRI GLPI
This software can report incidents on the MRI machine platform (microscopes, cytometers, tomograph RX)
• File management: SFTP server (see installation guide)
This interface allows you to manage your files, transfer them to your account or download them. The images will deleted after 2 years automatically.
• OMERO
This is a centralized software to manage your images. You can manipulate, share and store the images from this software.
• GLPI for MRI-NET
This software allows to report incidents or requests computer.
• Redmine
This software helps manage projects of the platform, some projects are public and some are private and not accessible to unauthorized persons.
Internally for facilities responsibles:
• GLPI for MRI-NET
This software allows to report incidents or requests computer.
• Dysfunction Editor
This application is used to declare and manage malfunctions on equipment in conjunction with the booking system.
• Autonomy Editor
This application allows you to manage user skills on equipment in conjunction with the booking system.
• Leave
Monitoring of staff leave the MRI platform.
• Quality
Document management system for quality
• Owncloud
Document management and online calendar for tracking quality of the platform. Sharing quality documents.
• Webmail
Our e-mail management system for staff with an address "mri.cnrs.fr".
Upright 2 Leica
Fluorescence -Brightfield
DIC
Tile-scan
Controlled by LAS X
10X Plan Apochromat 0.45 NA
20X Plan Apochromat 0.8 NA
40X Plan Apochromat 0.95 NA
40X Plan Apochromat 1.3 NA oil
63X Plan Apochromat 0.6-1.4 NA oil
Detector:
1 camera sCMOS Leica K5
(2048 x 2048 pixels, 6.5µm pixel size)
Excitation Source:
Lamp HXP120
L1 environment
Hoechst
GFP
Cy3
Texas Red
Cy5
Acquisition mode:
Multidimentional acquisition : 2D, 3D+time multicolor
Navigator Module for fast and easy mosaique, multiposition and focus correction