Loupe Olympus


Partner lab price : 5.36 €/hour
Public and private lab price: 22.32 €/hour
Associated technologies:
  • 2D imaging

    • Brief description:
    Stereomicroscope / fluorescence widefield

    Perfect for big samples observation or manipulation

    Color camera Sony MC5 Pro - 5Mpxl - pixel size = 3.45µm
    B&W images, color images, video/timelapse
    Filter cubes :
    - DAPI ex 350/50, em 460/50
    - TRITC ex 545/30, em 610/75
    - FITC ex 480/40, em 535/50

    Ideal operating temperature between 19 and 26°C

    L1 containment

    Posted in Stereomicroscopy

    LSR FORTESSA Becton Dickinson

    
    Partner lab price : 25.68 €/hour
    Public and private lab price: 50.75 €/hour
    Brief description:
    Flow cytometry cell analyzer - Becton Dickinson
    4 lasers, 2 physical parameters and 16 fluorescences

    Controlled by the FACSDiVa software (Becton Dickinson)
    Blue laser excitation 488nm (50mW) :
    Filter 1 (530/30BP) : FITC, AF488, GFP, CFSE, BB515
    Filter 2 (685LP - 710/50BP) : PerCP, PerCPCy5.5, PerCP-eF710

    Yellow-Green laser excitation 561nm (50mW) :
    Filter 3 (585/15BP) : PE, DsRed
    Filter 4 (600LP - 610/20BP) : mCherry, PI, PE-TexasRed, PE-CF594
    Filter 5 (635LP - 670/30BP) : PE-Cy5, 7-AAD
    Filter 6 (685LP - 710/50BP) : PE-Cy5.5
    Filter 7 (750LP - 780/60
    Violet laser excitation 400nm (50mW) :
    Filter 11 (450/50BP) : BD Horizon V450, Pacific Blue, AF405, DAPI, BV421
    Filter 12 (475LP - 525/50BP) : BD Horizon V500, Pacific Orange, AmCyan, Qdot525, BV510
    Filter 13 (595LP - 610/20BP) : Qdot605, BV605, eF605NC, eF625NC
    Filter 14 (630LP - 660/20BP) : Qdot655, BV650, eF650NC
    Filter 15 (685LP - 710/20BP) : Qdot700, BV711
    Filter 16 (750LP - 780/60BP) : BV786
    Red laser excitation 633nm (40mW) :
    Filter 8 (670/14BP) : APC, AF647
    Filter 9 (690LP - 730/45BP) : AF700, APC-R700
    Filter 10 (750LP - 780/60BP) : APC-Cy7, APC-H7, APC-AF750, APC-eF780
    High speed acquisition : 20 000 events/sec
    High sensitivity photomultiplier (PMT)

    Intracellular or cell-surface staining, fixed or alive cells

    L2 laboratory completely equiped : hood, centrifuge, pipets
    Ideal operating temperatures between 19 and 26°C

    Posted in 18 parameters analyzer

    Miltenyi Light Sheet Ultramicroscope Blaze

    
    Tarif établissement partenaire: 15.66 €/heure
    Tarif établissement public et privé : 55.41 €/heure
    Technologies associées :
  • Imagerie 3D

    • Brève description :
    Light sheet microscope for 3D imaging large cleared samples. The device is compatible with all clearing solutions (RI 1.33–1.56). It is controlled by ImSpector software.
    Objective
    2X MVPLAPO Olympus NA 0,5
    2 dipping cups, working distance 5,7mm or 10mm
    Optic manual zoom 0,63 to 6,3 i.e. total magnification 1.26x-12.6x

    Detector
    sCMOS camera
    2560 x 2160 pixels, pixels size : 6,5x6,5µm
    Acquisition type
    Multidimensional acquisitions, multi-color, tiles and multipositions
    Illumination sources and spectral selection:
    Double side illumination by light sheets (4-10µm thickness)
    Excitation laser 488 nm, emission filter 525/50
    Excitation laser 561 nm, emission filter 620/60
    Excitation laser 639 nm, emission filter 680/30
    Stainless steel cuvette compatible with all clearing solutions and water
    The sample is glued or screwed on the sample holder. Several samples can be imaged in sequential multiposition mode considering the maximum length of the sample holder is 52 mm.
    Contact us before starting your project
    This email address is being protected from spambots. You need JavaScript enabled to view it.
    

    Posted in Light Sheet

    Miltenyi MACS quant

    
    Partner lab price : 21.78 €/hour
    Public and private lab price: 59.44 €/hour
    Brief description:
    Flow cytometry cell analyzer, 3 lasers, up to 8 fluorescences and 2 physical parameters measured.
    Excitation/Detection:
    Violet laser excitation- 405 nm:
    - Filter 1V1 450/50BP
    - Filter 2 V2 525/50BP

    Blue laser excitation- 488 nm:
    - Filter 3 B1 525/50BP
    - Filter 4 B2 585/40BP
    - Filter 5 B3 692/75BP
    - Filter 6 B4 750LP

    Red laser excitation - 635 nm:
    - Filter 7 R1 692/75BP
    - Filter 8 R2 750LP
    Compatible vessels:
    Automatic sampler for 5/15/50mL tubes or any 96 wells plate. Up to 4 reagents may be automatically dispatched to the sample.

    Technical specifications:
    - acquistion speed up to 10 000 events per second
    Technology :
    Absolute cell counts are determined volumetrically, calculated automatically, and read out as events/μL for every sample and population. Cell counts are available in under a minute, eliminating the need for expensive counting beads or particles.


    Available techniques:
    Intracellular or cell-surface staining of fixed or alive (BSL1 only) cells.

    Please mind that the premises are biosafety level (BSL) one. A cell culture hood and incubator are available for any sample preparation.
    Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.

    Posted in 10 parameters analyzer

    MRI Center For Image Analysis

    Image and data analysis are an important part of imaging based research projects. MRI provides consulting, training and the hard- and software infrastructure needed for the analysis of imaging data. We also develop customized image analysis plugins for you.

    Contact: This email address is being protected from spambots. You need JavaScript enabled to view it.

    file info line2ABOUT MRI-CIA

    We offer comprehensive support for bioimage analysis, from the start of a research project to publication. The support we provide is tailored to each individual case and can include one-on-one software training or the creation of custom analysis tools.

    OPEN-DESK SESSION

    Do you have questions about image analysis? Do you need help with a script or macro? Are you planning to start an image analysis project with us? Then come join us at the next open-desk session.

    SOFTWARE AND TOOLS
    The facility provides access to     proprietary analysis software, open source software and tools developed in house. We also provide access to deep-learning solutions including the training on your own data on our machines.

    proprietary     open-source     deep-learning     tools 

    SCIENTIFIC WORKSTATIONS
    Our scientific workstations provide the hardware needed to run visualization and analysis software, including deep-learning solutions. You can make a reservation for a workstation here.

     crbm      dbs     igh    inm    isem    Lagaillarde     Lavalette 

     TRAINING AND CONSULTING

    Please contact the facility for training and consulting. The facility also provides freely accessible "Call for help" sessions. We regularly develop and animate image analysis related workshops within the context of the technological workshops of BioCampus Montpellier.

    SUCCESS STORIES

    Examples of successful MRI analysis projects.

     DATA-MANAGEMENT

     Data can be transferred between the sites of the facility via sFTP. For the scientific exploitation of the data the facility hosts a server OMERO.

     LINKS AND TUTORIALS

    Useful links to tutorials and other image analysis resources.
       

    Posted in English

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