Tarif établissement partenaire: 25.68 €/heure
Tarif établissement public et privé : 50.75 €/heure

This analyzer based on spectral cytometry is equipped with 4 lasers: blue, yellow, red, violet and allows a much more efficient separation of fluorochromes resulting in the possibility to increase the number of detectable colors simultaneously due to its 48 detectors (theorically 35 colors).

System located in L2 lab under hood

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Tarif établissement partenaire: 48.99 €/heure
Tarif établissement public et privé : 131.03 €/heure

This sorter based on spectral cytometry is equipped with 4 lasers: blue, yellow, red, violet and allows a much more efficient separation of fluorochromes resulting in the possibility to increase the number of detectable colors simultaneously due to its 48 detectors (theorically 35 colors).

This device is currently equipped with 2 nozzles of diameter 70 and 100 microns allowing to sort more or less quickly while preserving viability.
It allows sorting into tubes with up to 6 populations simultaneously, as well as 96-well plates and soon into 6, 12, 24 and 48-well plates.

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : €/hour Public and private lab price: €/hour

Flow cytometer
3 lasers
21 fluorescent channels
plate loader 96 wells (flat, U, V) and deep well

Ultra-violet Laser 355nm
Filter1 (405/30):UV405
Filter2 (525/40):UV525
Filter3 (675/30):UV675

Violet Laser 405nm
Filter4 (450/45):V450-PB
Filter5 (525/40):V525-KrO, BV510, Amcyan
Filter6 (610/20):V610, BV605
Filter7 (6¬¬60/10):V660, BV650
Filter8 (763/43):V763, BV785

Blue Laser 488nm
Filter9 (525/40):B525, FITC
Filter10 (610/20):B610, ECD, PI
Filter11 (690/50):B690, PC5.5

Yellow Laser 561nm
Filter12 (585/42):Y785, PE
Filter13 (610/20):Y610, mCherry, ECD
Filter14 (675/30):Y675, PC5, 7AAD
Filter15 (710/50):Y710, PC5.5
Filter16 (763/43):Y763, PC7

Red Laser 638nm
Filter17 (660/10):R660, APC
Filter18 (712/25):R712, APCA700
Filter19 (763/43):R763, APCA750

Infra Red Laser 808nm
Filter20 (840/20):IR840, AF790
Filter21 (885/40):IR885, Viakrome808

 

plate loader

L2 Lab

Prestations MRI sur les systemes d'imagerie Surveillance et maintenance

Partner lab price : 37.04 €/hour
Public and private lab price: 101.89 €/hour

3D imaging

Videomicroscopy

High resolution

FRAP photoactivation

TIRF

Super resolution 3D PALM / STORM

Super resolution 3D SIM

Inverted, fully-motorized, softWoRx software-controlled microscope for fluorescence and transmission imaging. This workstations allows either high speed (+TIRF, photomanipulation) or 3D-SIM & 2DPALM/STORM superresolution imaging.
405, 488, 561 & 642nm LASERs and LED for Brightfield/DIC imaging

Objectives:
60x Plan Apochromat 1.3 Sil
100x Plan SuperApochromat 1.4 Oil (DIC)

Detectors:
4 hight quantum efficiency photometrics Evolve 512B EMCCD cameras (512x512, effective pixels size of 132nm with 60x lens & 79nm with 100x lens).

Incubation:
Fully controlled environment including temperature, pH (CO2), oxygen concentration & hygrometry. Objective heater for 60x lens.

BSL1 environment

Available imaging techniques:
The device may be used in two distinct mode
- Highspeed imaging mode: 2 or 3D multiposition along time (up to a simultaneous 4 colors stack of 15 planes/sec). Hardware autofocus. Ratiometric FRET imaging, TIRF, Photomanipulation (FRAP/PA).

- Superresolution mode for a spatial resolution of 120x120x300nm (3D-SIM mode) and 2D PALM/STORM multiposition mode available.

Light sources:
405, 488, 561 (3 colors 3D-SIM) & 642nm LASERs excitation (4 colors highspeed imaging) and white LED (diascopic illumination)

Dichroic for fluorescence imaging:
East camera: 419-465nm
West camera: 500-550nm
North East camera: 581-618nm
North West camera: 664-704nm

Neutral density filters for LASER attenuation:
0.1%, 1%, 10%, 30%, 100%

Sample compatibility :
25mm coverslips (to be mounted in a chamber), 35mm glass-bottom dishes, thin-bottom Labtek chambers

Image quality:
For 3D-SIM imaging, sample preparation best practice should be followed. The coverslip thickness and mounting medium RI should be controlled. Spherical aberrations can be attenuated using different RI immersion oils.

Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour

2D imaging

3D imaging

Epifluorescent upright widefield microscope dedicated to 2D and 3D acquisition for fixed samples between slide and coverslip.
Fluorescence -Brightfield and Darkfield
DIC
Multi-dimensional acquisition

Controlled by NIS Element software

Objectives :
2X Plan Apo λ 0.1 NA
4X Plan Apo λ 0.2 NA
10X Plan Apo λ 0.45 NA
20X Plan Apo λ 0.75 NA
40X Plan Apo λ 0.95 NA
60X Plan Apo λ 1.4 NA oil
100X Plan Apo λ 1.45 NA oil

Excitation Source : Sola SE UV365

Filters:
Ex Dic Em
DAPI longpass UV-2A 330-380 400 420-800
GFP BanPass 446-486 495 500-550
GFP LongPass B-2A 450-490 500 515-800
Alexa 546 532-554 562 573-613
Texas Red 560-600 600 600-660

Detector : 1 Color camera DS-Ri2
4908*3264 px de 7.3µm

automatisation mode: XYZCT, multi-channels mosaic
custom-made protocol

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : 37.04 €/hour
Public and private lab price: 101.89 €/hour

2D imaging

3D imaging

Cuts acquisition automating

Spectral imaging

Multiphoton microscope
coupled to a vibratome
for imaging 3D
Drive by Zen (Zeiss)

Objectives :
10X Plan Apo 0.45 NA
20X Plan Apo 1.0 NA imm
40X LD C-Apo 1.1NA W
40X Plan Apo 1.4 NA oil
63X Plan Apo 1.4 NA oil

Excitation Source :
X-Cite 120 LED for observation

Lasers :
405, Argon (458, 488, 514), 561, 633 and Chameleon from 680 to 1080nm

Filter cubes:
DAPI / GFP / TX

Spectral confocal detection method

Detector :
2 PMTs
34 descanned spectral channels
additional GaAsP channel (BiG.2)

Acquisition mode:
multi-photon confocal -Imaging:
XYZ, XZY, lambda, time, multi-position or mosaic
-Automation with vibratome

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance

Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour

2D imaging

3D imaging

straight widefield epifluorescence microscope for 2D or 3D acquisition dedicated to fixed or alive samples between slide and coverslip

1 B&W camera and 1 color camera

Fluorescence, Brightfield, DIC

Apotome

Software : Zen by Zeiss

Objectives :
- 10X/0.45 Plan-Apo
- 20X/0.8 Plan-Apo
- 40X/1.3 Plan-Apo Oil
- 63X/1.4 Plan-Apo Oil
- 100X/1.4 Plan-Apo Oil

Filter cubes :
- DAPI ex G365, FT395, em BP445/50
- FITC ex BP475/40, FT500, em BP530/50
- CY3 / DsRed ex BP545/25, FT570, em BP605/70
- Texas Red ex BP560/40, FT585, em BP630/75
- Cy5 ex BP640/30, FT660, em BP690/50

Detectors :
- 1 B&W Camera (Zeiss Mrm)
pxl = 6.45µm, field = 1388x1040pxl
- 1 color camera (Zeiss ICc)

Apotome module to realise pseudoconfocal images (dedicated to fixed samples)

Z motorized microscope : 3D stacks and multichannel

L1 containment

Prestations MRI sur les systemes d'imagerie
Surveillance et maintenance