Widefield microscopy

Leica DM6B Thunder

Partner lab price: 
Public and private lab price: 
Associated technologies:
  • 2D imaging
  • 3D imaging

Short description:
Fully motorized, LASX software-driven, upright widefield epifluorescence microscope equipped with DIC optics.
Fluorescence and brightfield/DIC (w/b large field size camera).
For any multidimensional acquisition (X,Y,Z, lambda).
Objectives:
10X Plan Apochromat 0.45 NA
20X Plan Apochromat 0.8 NA
40X Plan Apochromat 1.3 NA oil DIC
63X Plan Apochromat 1.4NA oil DIC

Detector :
1 camera Leica K5 sCMOS monochrome
(2048 x 2048 pixels, 6.5µm pixel size)

Environment L1
Light sources :
LED3 Leica for episcopic illumination
White LED for diascopic illumination

Motorization:
Binocular tube, stage (X, Y and Z), fluorescence filter set, condenser and ,objective turrets.

Fluorescence filter sets:
DAPI Exc 405/60, FT465, Em470/40
GFP HE Exc 470/40, FT495, Em 525/50
Cy3 Exc 545/30, FT570, Em 610/75
Cy5 Exc 620/60, FT660, Em 700/75
Available acquisition techniques:
Any slide acquisition automation in both fluorescence and transmission modes.
Mosaic acquisition (full slide scan, slices on a slide scan) with user defined autofocus map.

Personnalization of protocols.
Image quality :
Quality and resolution improvement in real time or post processing mode with Leica THUNDER technology :
Instant Computational Clearing (ICC in 2D)
Large Volume Computational Clearing (LVCC in 3D)
Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.

Leica DM6B

Partner lab price :
Public and private lab price : 
Technologies:
- 2D imaging
- 3D imaging
Short description:
Fully motorized, LASX software-driven, upright widefield microscope equipped for epifluorescence and transmitted light microscopy.
For any multidimensional acquisition (X,Y,Z, lambda).
Motorized stage

 

Objectives:
10X Plan Apochromat 0.45 NA
20X Plan Apochromat 0.8 NA
40X Plan Apochromat 1.3 NA oil
63X Plan Apochromat 1.4 NA oil

Detector:
1 camera Leica K5 sCMOS mono
(2048 x 2048 pixels, 6.5µm pixel size)

Available acquisition techniques:
Any slide acquisition automation in both fluorescence and transmission modes (brightfield).
Mosaic acquisition (full slide scan, different objects on a slide), focus map (control of focus point number).

Personnalization of protocols.

Environment L1

Light sources:
PE300 Leica for episcopic illumination
White LED for diascopic illumination

Motorization:
Binocular tube, stage (X, Y and Z), revolver for fluorescence filter cubes, revolver for condenser, objective turret.

Fluorescence filter sets:
DAPI Ex 360/40, FT400, Em 470/40
GFP HE Exc 470/40, FT492, Em 525/50
Cy3 Exc 545/30, FT570, Em 610/75
Cy5 Exc 620/60, FT660, Em 700/75


Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.


Prestations MRI sur les systèmes d'imagerie
Surveillance et maintenance

Zeiss Axioimager Z2 Apotome

Partner lab price: 
Public and private lab price: 
Associated technologies:
  • 2D imaging
  • 3D imaging
  • Videomicroscopy
Brief description:
State-of-the-art, fully motorized, zen software-driven, upright widefield epifluorescence microscope equipped with an apotome slider and DIC optics.
Fluorescence and brightfield/darkfield/polarisation/DIC (w/b large field size camera).
For any multidimensional acquisition (X,Y,Z, lambda and t).
Objectives:
5X Plan Apochromat 0.16
10X Plan Apochromat 0.45 NA DIC
20X Plan Apochromat 0.8 NA DIC
40X Plan Apochromat 1.3 NA oil DIC
63X Plan Apochromat 1.4NA oil
100X Plan Apochromat 1.46 oil DIC

Detector:
1 ORCA-Flash4 LT Hamamatsu monochrome camera
(2048 x 2048 pixels, 6.5µm pixel size)

BSL1 environment
Light sources:
XCite 120LED, Zen-driven LED light source for episcopic illumination.
White Carl Zeiss LED source for diascopic illumination

Motorization:
Binocular tube, stage (X, Y and Z), fluorescence filter set turret, condenser turret, objective turret, apotome slider.

Fluorescence filter sets:
Hoechst
GFP HE
Cy3
Texas Red
Cy5
Polariser +/- a green filter for Picrosirius Red imaging.
Available acquisition techniques:
Any slide acquisition automation in both fluorescence and transmission modes. Images acquired with both color and w/b cameras are automatically realigned.
Mosaic acquisition (full slide scan, slices on a slide scan) with user defined autofocus mode and autofocus map.

User-defined protocols
Image quality:
Both axial resolution and contrast in epifluorescence mode may be improved using the Grid Projection Illumination technique (aka "apotome"). The grid lines spacing is automatically set (motorized apotome slide). The spatial resolution may be further improved using deconvolution (Huygens or Zen software).
Inquiries may be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.