Public and private lab price :
2D imaging
3D imaging
FRAP Photoactivation Photoconversion
FRET-FLIM
Spectral imaging
Inverted confocal microscope, LAS X software driven
LASERs : White light laser WLL + 405 nm diode )
3 détectors (photon counting mode, analog or digital)
Simultaneous acquisition of intensity and lifetime images and multichannel
Multi-dimensional acquisition (X,Y,Z, spectral and time)
Illumination source for widefield observation with eyepieces: HXP120 lamp
Fluorescence filters set:
DAPI (Ex BP360/40 / Em LP425)
GFP (Ex BP470/40 / Em BP 525/50)
Dsred ET k (Ex BP546/11 /Em BP605/75)
Objectives
63x HCX PL APO 1.4 oil CS2
40X HCX PL APO 1,1 corr water CS2
20X HC PL APO 0,75 CS
10X HC PL Fluotar 0,3
DIC available for 40Xw et 63Xoil
Lasers:
Diode 405 nm,
Pulsed WLL 80MHz (440-790nm)
Detectors :
HyDS, HyDX, HyDX, PMTtrans
tauSense tool (mean lifetime) :
TauContrast (average photon arrival time measurement per pixel, on the fly)
TauGating (to design several temporal windows simultaneously)
Tauscan & TauSeparation (fluorescence splitting based on lifetime, on the fly)
TauInteraction (FRET efficency measurement)
FALCON tool (FAst Lifetime imaging CONtrast) : quantification by pixel