Widefield microscopy

Upright 2 Zeiss Apotome

Partner lab price: 
Public and private lab price: 
Associated technologies:
  • 2D imaging
  • 3D imaging
Brief description:
Upright widefield epifluorescence microscope ideal for 2D or 3D acquisitions followed by deconvolution, for fixed samples between slide and coverslip. Apotome module for optical sectioning.

Multi-dimensional acquisition

Acquisition software: ZEN Blue 3.2
website Apotome
Objectives:
5X EC Plan Neofluar 0.15 NA
10X EC Plan Neofluar 0.3 NA
20X Plan Apochromat 0.8 NA
40X EC Plan Neofluar 1.3 NA oil
63X Plan Apochromat 1.4 NA oil
100X alpha Plan Apochromat 1.46 NA oil
Camera: Hamamastu Orca flash 4.0 - B&W, 16-bits, 2048x2048 pixels, pixel size @6.5 µm
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Excitation source: HXP120V Lamp

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Filter cubes:
Hoechst
GFP
Cy3
Texas Red
Cy5
FluorGold
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Type of acquisition:
Automated on a slide: XYZ, multichannel, mosaic
Customizable protocol
Mouse spine - MRI 2007 Image Contest 2nd prize
Transverse section of mouse spine at P0 stage, with spinal cord in the center and dorsal root ganglia on either side: Ret (Magenta), NF200 (Cyan).
Steve Bourane, Institute for Neurosciences of Montpellier (INM), Montpellier

Upright Leica Thunder

Partner lab price:
Public and private lab price: 
Technologies:
- 2D imaging
- 3D imaging
Short description:
Upright widefield epifluorescence microscope ideal for 2D or 3D acquisitions followed by deconvolution, for fixed samples, calcium imaging, eye imaging in vivo.

Image quality improvement with software technology THUNDER.
Multi-dimensional acquisition
Acquisition software: LASX

 

Objectives:
1.6x/0.05 HC PL Fluotar
10X/0.32 HC PL Fluotar
16X/0.60 HC Fluotar L IMM CORR
20X/0.55 HC PL Fluotar
20X/0.80 HC PL APO
40X/0.95 HC PL APO CORR
63X/1.40 Oil HC PL APO

20X/0.80 Oil HC PL Fluotar (on demand for Fura)

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Light sources:
- Fluorescence: pe-800 fura (wavelengths (nm): 340, 380, 435, 470, 500, 550, 580, 635)
- Transmitted light: white LED

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Filter cubes: 3 multiband cubes
- QL1: DAPI, GFP, AF594, AF647
- CYR: CFP, YFP, RFP
- Fura: 340/380 (calcium probe)

Cameras:
- for fluorescence: B&W K8 sCMOS 4.2MP (2048x2048, pixel size = 6.5µm)
- for transmitted light: K3C color CMOS 6.3 MP (3072x2048, pixel size = 2.4 µm)

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Acquisition types:
- Automated on samples, from 1 to 4 slides: XYZT, multichannel, mosaics
- Navigator module and external filter wheel to improve acquisition speed
- Calcium imaging
Customizable protocol

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Improvement of image quality with THUNDER software technology, during or after acquisition

Prestations MRI sur les systèmes d'imagerie
Surveillance et maintenance