Confocal microscopy

CONFOCAL ZEISS LSM980 AIRYSCAN 8Y

Partner lab price: 
Public and private lab price: 
Associated technologies:
  • 3D imaging
  • High resolution
  • FRAP photoactivation
  • Spectral imaging

Brève description :
Inverted confocal microscope for 3D imaging of fixed sample (slide-coverslip) or live sample (bottom glass coverslip dish). Improvment of spatial resolution thank to the Airyscan 2 module and speed of acquisition thank to the AiryScan Multiplex 4Y & 8Y.
Objectives:
20X Plan Apo 0.8NA
40X Plan Apo huile 1.3NA
63X Plan Apo huile 1.4NA
40X Plan Apo eau 1.1NA
Detectors:
-2 PMT for fluorescence
-1 GaAsP spectral detector 32 chanels
-1 PMT for transmited light
-1 AiryScan2 detector
Illumination source for ocular widefield visualisation :
Colibri 5
Cube for widefield visualisation : 3 bands DAPI/GFP/CY3

Environment control: Temperature, CO2 and humidity

Excitation sources for confocal mode :
Laser diodes 405nm, 445nm, 488nm, 515nm, 561nm and 639nm
Acquisition modes:
Zeiss Zen Blue Software
-multi-modal acquisition: XYZ, spectral, time, multi-positions & mosaic
-« super-resolution » imaging for fixed samples
-Live and « super-resolution » imaging with the multiplex 4Y & 8Y AiryScan
-AI Sample Finder module
-FRAP module

Leica SP8-UV confocal microscope

Partner lab price: 
Public and private lab price: 
Associated technologies:
  • 3D imaging
  • Videomicroscopy
  • FRAP photoactivation
  • Ratiometric FRET
  • DNA damage
  • Spectral imaging

Brief description:
State-of-the-art fully motorized, LAS software-driven, inverted confocal microscope (+UV) for epifluorescence and transmission imaging.
Fluorescence (355, 405, Argon, 561 and HeNe 633 LASERs) and brightfield/DIC.
Multidimensional acquisitions (X,Y,Z, spectral and time), F-techniques, incubation
Objectives:
10X Plan Apochromat 0.4 NA Imm
20X Plan Apochromat 0.75 NA Imm Corr
40X Plan Apochromat 1.3 NA oil DIC
63X Plan Apochromat 1.4 NA oil DIC
63X Plan Apochromat 1.2W UVIS + water dispenser

Detectors:
2 cooled regular multialkaline PMTs
1 transmission PMT
3 cooled HyDs (GaAsP photocathode) for low noise photon counting imaging.

BSL1 Environment
Illumination light source for widefield observation :
Fibred metal halide arc source for episcopic illumination
100W tungsten bulb for diascopic illumination

Motorization:
Binocular tube, X, Y and Z stage, fluorescence filter set turret, transmission mirror, objective turret

Fluorescence filter sets for widefield observation:
Hoechst
GFP
Rhodamine LP
Cy5
Available acquisition techniques:
Excitation with 355, 405, 458, 476, 488, 514, 561 & 633nm lines.
2/3D mosaic acquisition, spectral separation, timelapse analysis. In-focus repositioning.
Any photoactivation/destruction technique (FRAP, PA, FLIP), ratiometric FRET experiment, UVA induced DNA-damange on sensitized cells.
Screening and on the fly analysis (Matrix screener)

User-defined protocols
Image quality:
Confocal technology allows improved spatial resolution and contrast compared to widefield imaging. Spatial resolution may be further improved using deconvolution algorithms (Huygens).

Sensitivity:
The HyD design allows better sensitivity and SNR compared to regular PMT and a less noisy photon counting imaging.
The fast resonant scanner decrease excitation-related toxicity.
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Microscope Biphoton Bruker ULTIMA 2Pplus

Partner lab price :
Public and private lab price : 
Technologies :
2D imaging
3D imaging
FRAP Photoactivation Photoconversion
Laser ablation
Short description :
Bruker upright microscope coupled with two Coherent lasers, enabling cellular and tissue imaging of live or fixed samples, and integrated with electrophysiology. Prairie View acquisition software.

 

Objective: 20X Olympus XLUMPLFLN (N.A. 1) water immersion, WD 2 mm.
Detectors: 3 GaAsP NDD detectors.

Acquisition modes: XYZ multi-photon confocal imaging, time-course, multi-position or mosaic. Photostimulation/photoactivation experiments. The system is equipped with a galvanometric scanner and a resonant scanner. Multi-photon imaging can be coupled with electrophysiological recordings.
Customisable protocols.

Excitation sources: Chameleon Discovery NX (Coherent) dual-line laser: one tunable line in the 660–1320 nm range and one fixed line at 1040 nm. A delay line enables the temporal synchronisation of the tunable and fixed laser beams.

Spectral configuration:
For two-photon imaging, an LP 565 dichroic filter combined with BP 525/70 and BP 595/50 filters, and an LP 495 dichroic filter combined with a BP 485/25 filter.
Full-field observation: fluorescence and transmission. A GFP cube (470/40, 495LPXR, 525/50) and a DsRed cube (545/30, 570LP, 620/60) combined with a Retiga Electro CCD camera for observation and navigation across the sample.

Sample holders: Slice Mini bathchamber II (Luigs&Neumann).
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Prestations MRI sur les systèmes d'imagerie
Surveillance et maintenance

Nikon spinning disk CSU-W1

 

Partner lab price :
Public and private lab price : 
Technologies :
2D imaging
3D imaging
FRAP Photoactivation Photoconversion
Videomicroscopy
Short description :
Nikon inverted microscope coupled with a Yokogawa CSU-W1 spinning-disc head, designed for 3D confocal imaging of live samples. The system is controlled by NIS software.
Motorised stage.

 

Objectives
4x PlanApo Lambda D (N.A. 0.2) air
25x Plan Apo Lambda S (N.A. 1.05) silicone immersion
40x Plan Apo Lambda D (N.A. 0.95) air immersion
60x Super Plan Fluor ELWD (N.A. 0.70) air immersion
60x Plan Apo Lambda D (N.A. 1.42) oil immersion
100x Plan Apo Lambda D (N.A. 1.45) oil immersion
100x Plan Apo Lambda S (N.A. 1.35) silicone immersion

Detectors:
Hamamatsu Orca Fusion BT sCMOS camera (2304 x 2304, pixel size 6.5 µm)
Andor iXon Life 888 EMCCD camera (1024 x 1024, pixel size 13 µm)

Environmental control: temperature and CO₂
Acquisition types: multi-dimensional transmitted light, DIC and confocal fluorescence acquisitions: XYZ, multi-channel, multi-position, mosaic, multi-well, time-lapse. TIRF and FRAP (ILAS 2).
Focus tracking (PFS)
Customisable protocols

Excitation sources: 405 nm (120 mW), 445 nm (100 mW), 488 nm (200 mW), 515 nm (100 mW), 561 nm (150 mW) and 638 nm (200 mW) lasers.

Spectral configuration:
Dichroic filters in the head: triple-band 445/515/561, quad-band 405/488/561/640
Emission filters for the CMOS camera: DAPI (447/60), GFP (525/30), YFP (524/27), Cy3 (600/52), Cy5 (708/75), Dual GFP/dsRed (532/610)
Emission filters for the EMCCD camera: DAPI (447/60), CFP (472/30), GFP (525/30), YFP (524/27), Cy3 (600/52), Cy5 (708/75), Dual GFP/dsRed (532/610)

Compatible sample holders: 35mm boxes with #1.5 glass cover slip base, #1.5 glass cover slip mounted on a slide, multi-well plate with #1.5 glass cover slip base.

For further information, please contact This email address is being protected from spambots. You need JavaScript enabled to view it.

 

 

 

Prestations MRI sur les systèmes d'imagerie
Surveillance et maintenance