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Partner lab price : 15.66 €/h
Public and private lab price : 55.41 €/h
Technologies:
- 2D imaging
- 3D imaging
- Laser microdissection
Short description:
- Fully motorized epifluorescent upright widefield microscope, ideal for all 2D/3D acquisitions of fixed samples mounted between slide and coverslip.
- Thunder module for noise reduction and adaptive deconvolution.
- Navigator module for mosaic imaging.
- Phase contrast imaging.
- Laser microdissection module for genomics (DNA), transcriptomics (mRNA, RNA), proteomics, and metabolomics.

 

Objectives:
UVI 5x/0.12 (compatible with microdissection)
HC PL FLUOTAR 10x/0.32 PH1 (compatible with microdissection)
HC PL FL L 40x/0.60 CORR.0–2 mm PH2 XT / WD 1.9–3.3 mm (compatible with microdissection)
HC PL Fluotar L 63x/0.70 CORR.0.1–1.3 mm PH2 / WD 1.8–2.6 mm (compatible with microdissection)
HC PL APO 20x/0.80 / WD 0.4 mm
HC PL APO 40x/0.95 CORR.0.11–0.23 mm / WD 0.17 mm
HC PL APO 40x/1.30 OIL
HC PL APO 63x/1.40–0.60 OIL / WD 0.14 mm

Detectors:
- LEICA K3C (color sCMOS camera – pixel size 2.4 × 2.4 µm²) – compatible with microdissection
- LEICA K8 (sCMOS camera with 95% QE – pixel size 6.5 × 6.5 µm²)

LAS X Acquisition Software
Multidimensional 2D, 3D + time, multicolor acquisition with a 4-slide holder.
Navigator module for rapid mosaic and multiposition imaging with focus correction.
Contrast enhancement using Thunder ICC (2D) and LVCC (3D) denoising technology with adaptive deconvolution.

LMD Acquisition Software (Dedicated to Microdissection)
Laser microdissection module enabling contact-free, contamination-free dissection from single cells to tissue samples.
Sample collection by gravity-assisted transfer and multi-collector systems.
Phase contrast imaging available at 10x, 40x, and 63x magnifications.

Fluorescence and Transmitted Light Illumination Sources:
- PE300 LED for fluorescence imaging.
- White LED for transmitted light imaging.
- Laser 349nm for microdissection / Pulse frequency 10–500–50,000 Hz / Maximum pulse energy 120 µJ

Filter Cubes:
DAPI for LMD applications: Ex 360/40, FT 400, Em 470/40
GFP for LMD applications: Ex 470/40, FT 495, Em 525/50
Cy3 for LMD applications: Ex 535/50, FT 564, Em 610/75
Cy5 for LMD applications: Ex 620/60, FT 660, Em 700/75

Environmental Control: Temperature and CO2 control.
Biosafety Level 2 (BSL-2) environment.