Multi-photon Zeiss LSM 7MP OPO


Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour
Associated technologies:
  • 2D imaging
  • 3D imaging
  • SHG / THG
  • In vivo imaging

    • Brief description:
    Multi-photon microscope on upright stand, ideal for 3D acquisitions on live samples, in vivo on small animals, thick cleared or not cleared samples.

    Fluorescence
    Label-free imaging: SHG, THG, CARS
    Multi-dimensional acquisition

    Acquisition software: Zen Black 2012
    Objectives:
    10X EC Plan-Neofluar 0.3 NA
    20X Plan Apochromat 1.0 NA Water
    20X Plan Neofluar 1.0 NA Clr Korr RI = 1.45 +/- 0.03 (on request)
    40X Plan Apochromat 1.0 NA Water
    Detection:
    2 PMTs NDD
    2 BiG

    Spectral separation of fluorochromes: several available configurations, from blue to far red

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    Environmental control (T°)
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    To locate the sample with eyepieces:
    Excitation source: HXP120V lamp
    Filter cubes: DAPI / GFP / Rhod

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    Laser sources:
    - Chameleon Ultra II: 680 to 1080 nm
    - Chameleon Compact OPO (optical parametric oscillator): 1000 to 1300 nm
    - - -
    Types of acquisition:
    - multi-modal imaging: XYZ, time lapse, multichannel, mosaïc, multi-positions
    - imaging on small animal
    - fluorescence
    - SHG: second harmonic generation
    - THG: third harmonic generation
    - CARS: Coherent Anti-Stokes Raman Scattering