Confocal ZEISS LSM880 FastAiryscan


Tarif établissement partenaire: 37.04 €/heure
Tarif établissement public et privé : 101.89 €/heure

Brève description :
Inverted confocal microscope for 3D imaging on fixed (slide/coverslip) or live samples (bottom glass petridish).
Improvement of spatial resolution and speed with Airyscan and FastAiryscan module.
Microscope equiped with DIC and autofocus
Objectives :
10X/0.3 Plan-neofluar
20X/0.8 Plan-apochromat
40X/1.1 W C-apochromat (long distance) DIC
40X/1.4 Oil Plan-apochromat DIC
63X/1.4 Oil Plan-apochromat DIC

Occular fluorescence with HXP120
filter cubes : DAPI, GFP, Cy3
Lasers :
Diode 405
Laser Argon 458, 488, 514
DSSP 561
Laser He/Ne 633

Detectors :
- 2 PMT fluorescence
- 1 GaAsP (high sensitivity)
- 1 PMT Transmission
- 1 Airyscan detector (32 GaAsP)

Acquisition software : ZEN black (.czi files format)
- multi modal aquisitions : XYZ, time, spectral, multiposition, mosaic
- "super-resolution" imaging with Airyscan and FastAiryscan module
- hardware solution for autofocus (Definite focus)
- FRAP, FRET, Photoactivation module
- Experiment designer module


Spinning disc Nikon Ti Andor CSU-W1


Partner lab price : 15.66 €/hour
Public and private lab price: 55.41 €/hour
Associated technologies:
  • 3D imaging
  • Videomicroscopy
  • In vivo imaging

    • Brief description:
    ANDOR confocal spinning disk on an inverted NIKON microscope (Ti Eclipse)

    Dedicated for live samples observations on multiweel or petridishes

    XYZ motorized microscope and temperature and CO2 control, focus control

    B&W camera Zyla 4.2, software : iQ3 by Andor
    Objectives :
    - 4x/0.13
    - 10x/0.45
    - 20x/0.75 DIC
    - 40x/1.15 Water
    - 60x/1.4 Oil DIC
    - 100x/1.45 Oil DIC

    Fluorescence filters :
    - DAPI ex BP377/50, DM409
    - FITC ex BP482/35, DM506, Em BP536/40
    - Texas Red ex BP562/40, DM593, Em BP624/40
    - Cy5 ex BP628/40, DM660, Em BP692/4

    Lasers :
    - 405
    - 488
    - 561
    - 640

    QUAD filters for emission :
    425-470nm - 505-542nm - 578-623nm - 660-750n
    Spinning disk Yokogawa head CSU-W1 equiped with two dichroïcs (3 bands 405/488/561 and 4 bands 405/488/561/640)

    B&W Caméra Zyla 4.2
    field = 2048x2048pxl, pixel size = 6.5µm
    IQ3 software
    Motorized microscope in XYZ : 3D stack, timelapse with variable frequency, multiposition, mosaïque, multichannel (combined dimension for handmade experimentations)

    Ideal operating temperature between 19 and 26 °C

    L2 Containment